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Dengue Engineering

September 3, 2023

Science Advances, 2021 Designed, highly expressing, thermostable dengue virus 2 envelope protein dimers elicit quaternary epitope antibodies Stephan Kudlacek, Stefan Metz, …. , Aravinda de Silva, Brian Kuhlman   Dengue virus (DENV) is a worldwide health burden, and a safe … Read more

Enzyme Turbocharging

September 3, 2023

Nature Chemical Biology, 2023, 19(4):460-467 Designer installation of a substrate recruitment domain to tailor enzyme specificity. Park R, Ongpipattanakul C, Nair SK, Bowers AA, Kuhlman B.   Promiscuous enzymes that modify peptides and proteins are powerful tools for labeling biomolecules; … Read more

Light Controllable Nuclear Export

May 17, 2016

Nat Chem Biol. 2016 Apr 18 Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Yumerefendi H, Lerner AM, Zimmerman SP, Hahn K, Bear JE, Strahl BD, Kuhlman B. We engineered a photoactivatable system for rapidly and reversibly exporting proteins from … Read more

Protein SEWING

May 17, 2016

Science. 2016;352(6286):687-90 Design of structurally distinct proteins using strategies inspired by evolution. Jacobs TM, Williams B, Williams T, Xu X, Eletsky A, Federizon JF, Szyperski T, Kuhlman B.   Natural recombination combines pieces of preexisting proteins to create new tertiary structures and … Read more

A Modified H-bond Potential for Rosetta

January 22, 2016

  J Chem Theory Comput. 2015;11(2):609-622. A Combined Covalent-Electrostatic Model of Hydrogen Bonding Improves Structure Prediction with Rosetta. O’Meara MJ, Leaver-Fay A, Tyka M, Stein A, Houlihan K, DiMaio F, Bradley P, Kortemme T, Baker D, Snoeyink J, Kuhlman B. … Read more

Design of a Light Activatable Dimer

August 27, 2015

Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins Gurkan Guntas, Ryan A. Hallett, Seth P. Zimmerman, Tishan Williams, Hayretin Yumerefendi, James E. Bear, Brian Kuhlman Proc Natl Acad Sci U S A. 2015, … Read more

A New Bispecific Antibody Platform

October 1, 2014

Nature Biotechnolgy. 2014 v32(2):191-8. Generation of bispecific IgG antibodies by structure-based design of an orthogonal Fab interface. Lewis SM, Wu X, Pustilnik A, Sereno A, Huang F, Rick HL, Guntas G, Leaver-Fay A, Smith EM, Ho C, Hansen-Estruch C, Chamberlain … Read more