Predicting mutation effects on protein stability
Transfer learning to leverage larger datasets for improved prediction of protein stability changes. Proc Natl Acad Sci U S A. 2024 Dieckhaus H, Brocidiacono M, Randolph NZ, Kuhlman B. Amino acid mutations that lower a protein’s thermodynamic stability are implicated … Read more
Dengue Engineering
Science Advances, 2021 Designed, highly expressing, thermostable dengue virus 2 envelope protein dimers elicit quaternary epitope antibodies Stephan Kudlacek, Stefan Metz, …. , Aravinda de Silva, Brian Kuhlman Dengue virus (DENV) is a worldwide health burden, and a safe … Read more
Enzyme Turbocharging
Nature Chemical Biology, 2023, 19(4):460-467 Designer installation of a substrate recruitment domain to tailor enzyme specificity. Park R, Ongpipattanakul C, Nair SK, Bowers AA, Kuhlman B. Promiscuous enzymes that modify peptides and proteins are powerful tools for labeling biomolecules; … Read more
Light Controllable Nuclear Export
Nat Chem Biol. 2016 Apr 18 Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Yumerefendi H, Lerner AM, Zimmerman SP, Hahn K, Bear JE, Strahl BD, Kuhlman B. We engineered a photoactivatable system for rapidly and reversibly exporting proteins from … Read more
Protein SEWING
Science. 2016;352(6286):687-90 Design of structurally distinct proteins using strategies inspired by evolution. Jacobs TM, Williams B, Williams T, Xu X, Eletsky A, Federizon JF, Szyperski T, Kuhlman B. Natural recombination combines pieces of preexisting proteins to create new tertiary structures and … Read more
A Modified H-bond Potential for Rosetta
J Chem Theory Comput. 2015;11(2):609-622. A Combined Covalent-Electrostatic Model of Hydrogen Bonding Improves Structure Prediction with Rosetta. O’Meara MJ, Leaver-Fay A, Tyka M, Stein A, Houlihan K, DiMaio F, Bradley P, Kortemme T, Baker D, Snoeyink J, Kuhlman B. … Read more
Design of a Light Activatable Dimer
Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins Gurkan Guntas, Ryan A. Hallett, Seth P. Zimmerman, Tishan Williams, Hayretin Yumerefendi, James E. Bear, Brian Kuhlman Proc Natl Acad Sci U S A. 2015, … Read more
A New Bispecific Antibody Platform
Nature Biotechnolgy. 2014 v32(2):191-8. Generation of bispecific IgG antibodies by structure-based design of an orthogonal Fab interface. Lewis SM, Wu X, Pustilnik A, Sereno A, Huang F, Rick HL, Guntas G, Leaver-Fay A, Smith EM, Ho C, Hansen-Estruch C, Chamberlain … Read more