Welcome!  We are in the Department of Biochemistry and Biophysics at the University of North Carolina, Chapel Hill. We use a combination of computational and experimental methods to design proteins.

Currently we are focusing on a variety of design goals including the creation of novel protein-protein interactions, protein structures and light activatable protein switches. Central to all of our projects is the Rosetta program for protein modeling. In collaboration with developers from a variety of universities, we are continually adding new features to Rosetta as well as testing it on new problems.

Please see our research page for more information.

Recent Highlights

Light Controllable Nuclear Export

Nat Chem Biol. 2016 Apr 18 Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Yumerefendi H, Lerner AM, Zimmerman SP, Hahn K, Bear JE, Strahl BD, Kuhlman B. We engineered a photoactivatable system for rapidly and reversibly exporting proteins from … Continued

Protein SEWING

Science. 2016;352(6286):687-90 Design of structurally distinct proteins using strategies inspired by evolution. Jacobs TM, Williams B, Williams T, Xu X, Eletsky A, Federizon JF, Szyperski T, Kuhlman B.   Natural recombination combines pieces of preexisting proteins to create new tertiary structures and … Continued

A Modified H-bond Potential for Rosetta

  J Chem Theory Comput. 2015;11(2):609-622. A Combined Covalent-Electrostatic Model of Hydrogen Bonding Improves Structure Prediction with Rosetta. O’Meara MJ, Leaver-Fay A, Tyka M, Stein A, Houlihan K, DiMaio F, Bradley P, Kortemme T, Baker D, Snoeyink J, Kuhlman B. … Continued

Design of a Light Activatable Dimer

Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins Gurkan Guntas, Ryan A. Hallett, Seth P. Zimmerman, Tishan Williams, Hayretin Yumerefendi, James E. Bear, Brian Kuhlman Proc Natl Acad Sci U S A. 2015, … Continued